甲型流感病毒IgG基因捕獲法檢測試劑盒

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甲型流感病毒IgG基因捕獲法檢測試劑盒

基因捕獲法是利用隨機插入突變進行基因敲除的新型方法。通?；虿东@載體還包括一個無啟動子的報道基因，通常是neo 基因，neo 基因插入到ES 細胞染色體組中，并利用捕獲基因的轉錄調控元件實現表達的ES 克隆可以很容易地在含G418 的選擇培養基中篩選出來，從理論上講，在選擇培養基中存活的克隆應該100%地含有中靶基因。中靶基因的信息可以通過篩選標記基因側翼cDNA或染色體組序列分析來獲得。

基因捕獲法是利用隨機插入突變進行基因敲除的新型方法。通?；虿东@載體還包括一個無啟動子的報道基因，通常是neo 基因，neo 基因插入到ES 細胞染色體組中，并利用捕獲基因的轉錄調控元件實現表達的ES 克隆可以很容易地在含G418 的選擇培養基中篩選出來，從理論上講，在選擇培養基中存活的克隆應該100%地含有中靶基因。中靶基因的信息可以通過篩選標記基因側翼cDNA或染色體組序列分析來獲得。

用常規方法進行基因敲除研究需耗費大量的時間和人力，研究者必須針對靶位點在染色體組文庫中篩選相關的染色體組克隆，繪制相應的物理圖譜，構建特異性的基因敲除載體以及篩選中靶ES 細胞等，通常一個基因剔除純合子小鼠的獲得需要一年或更長的時間。面對人類基因組計劃產生出來的巨大的功能未知的遺傳信息，傳統的基因敲除方法顯得有些力不從心。因此，基因捕獲法應運而生，利用基因捕獲可以建立一個攜帶隨機插入突變的ES 細胞庫，節省大量篩選染色體組文庫以及構建特異打靶載體的工作及費用，更有效和更迅速地進行小鼠染色體組的功能分析。
此方法的缺點是只能剔除在Es 細胞中表達的基因．單種的細胞類型中表達的基因數目約為I04，現在的基因捕獲載體從理論上來講應能剔除所有在ES細胞表達的基因，因此，在ES 細胞中進行基因捕獲還是大有可為的。用基因捕獲法進行基因剔除的另一個缺點是無法對基因進行精細的遺傳修飾。
 

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Gene capture is a novel method of gene knockout using random insertions. Generally, the gene capture vector also includes a promoterless reporter gene, usually a neo gene. The neo gene is inserted into the genome of the ES cell and an ES clone expressing the transcriptional regulatory element of the capture gene can be easily expressed in a G418-containing The selection medium is screened, and theoretically, the clone that survives in the selection medium should contain 100% of the target gene. The target gene information can be obtained by screening the flanking cDNA or genomic sequence analysis of the marker gene.

Gene capture is a novel method of gene knockout using random insertions. Generally, the gene capture vector also includes a promoterless reporter gene, usually a neo gene. The neo gene is inserted into the genome of the ES cell and an ES clone expressing the transcriptional regulatory element of the capture gene can be easily expressed in a G418-containing The selection medium is screened, and theoretically, the clone that survives in the selection medium should contain 100% of the target gene. The target gene information can be obtained by screening the flanking cDNA or genomic sequence analysis of the marker gene.

Gene knockout studies using conventional methods take a considerable amount of time and manpower. Researchers have to screen for the relevant genomic clones in the genomic library against the target site, map the corresponding physical maps, construct specific knockout vectors, and Screening of target ES cells and the like usually takes one year or more to obtain a gene knockout homozygous mouse. In the face of huge untapped genetic information generated by the Human Genome Project, traditional methods of gene knock-outs seem a bit powerless. Therefore, the gene capture method came into being, the use of gene capture can create a library of ES cells carrying random insertion mutations, saving a large number of screening of the genome library and the construction of specific targeting vector work and costs more efficient and more prompt mouse chromosome Group functional analysis.
The disadvantage of this method is that only the genes expressed in Es cells can be excluded. The number of genes expressed in a single cell type is about 104, and now the gene capture vector is theoretically able to knock out all the genes expressed in ES cells. Therefore, gene capture in ES cells is still promising. Another drawback of gene knockout using gene trapping is the inability to fine-tune genes for genetic modification.